BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat. Impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies.

Pure stocks were obtained from degracadoras isolated by streaking, verifying their homogeneity by Gram staining and through the examination of culture characteristics. In addition, other authors have shown that the biotransformation of asphaltenes and resins leads to the accumulation of simpler saturated and aromatic derivates, increasing the concentration of these fractions [53, 54].

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Another two strains belonged to the genera Acinetobacter and Marinomonasrespectively. These cultures were then further incubated for seven deyradadoras under the same conditions, at the end of which the subculturing cycle was repeated once more. The cultures were examined every 24 h, selecting all strains exhibiting vigorous growth within a degradadorad day period for further study.

Most research on bioremediation technology has focused therefore on aerobic heterotrophic bacteria, due not only to the taxonomic diversity of hydrocarbon-degrading representatives from this group, but to their ability to use xenobiotic compounds as carbon source in pure cultures [3]. All three strains under examination produced a notable decrease in the concentration of asphaltenes, compared to the control.

This decrease in isoprenoids is a telltale sign of effective biodegradation; whereas strain F1FLC exhibits values above those of the abiotic control.


Microbiol Mol Biol Rev. The degradation potential exhibited bacyerias these new isolates warrants further studies on their possible application to decontaminate coastal environments affected by oil spills. Hydrocarbon determinations Hydrocarbon determinations were performed at day degradaadoras After concluding the three subculture cycles, 0. At the end, 18 strains representing each unique combination of these parameters were chosen to be subjected to the selection process.

Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil. Microbial degradation of hydrocarbons in the environment.

Publications reporting the presence of this genus in hydrocarbon-contaminated ecosystems and describing its hydrocarbon-degrading abilities have appeared in the literature from the early nineties [17], although its numbers have increased as of late [19, 24, 25, 31, 32].

Measures taken after the Exxon Valdez spill included the addition of nutrients in the form of fertilizers Inipol EAP 22 and Customblerwhich increased the rate of oil removal by three-fold [6].

These compounds did not mineralize completely, accumulating instead as intermediary metabolites consisting of linear chains and aromatic compounds. In addition, it must be noted that synthesis of the enzyme complexes required to degrade the heavier fractions is not induced until lighter fractions have been exhausted, following the principle of maximum cellular economy metabolic regulation. Five replicates were used per sample. Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments.

Some species of this genus have been isolated from marine environments contaminated with hydrocarbons [5, 17, 28]. Had hydrocarbon composition been determined at that point, we would have most likely found practically identical levels of asphaltenes and resins in crudes treated with the strains under examination and in their controls, significantly decreased levels of saturated hydrocarbons in the former, and only a small drop in the concentration of aromatic hydrocarbons, since the bulk of their degradation takes place after 21 days.


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Asphaltenes were precipitated with n-pentane. Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane alk and naphthalene nah catabolic pathways. A Mycobacterium strain with extended capacities for degradation of gasoline hydrocarbons. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants.

The obtained isolates were then subjected to selection in Bushnell-Haas medium supplemented with a heavy crude oil, selecting three strains able to degrade this hydrocarbon mixture within a period of seven days.

BACTERIAS DEGRADADORAS DE HIDROCARBUROS by Mirelly Katherine Diaz Gamarra on Prezi

However, and despite the higher propensity of n-alkanes for oxidation [35, 49], no differences in biodegradation percentages were detected degradaoras saturated hydrocarbons, asphaltenes and monocyclic aromatic hydrocarbons after 45 days. F9S and Bacillus sp.

Biodegradation of crude oil contaminating marine shorelines and freshwater wetlands.

We were unable to find previous mentions in the literature of the presence of these microorganisms in hydrocarbon-contaminated environments. Total petroleum hydrocarbon TPH degradation was evaluated through SARA analysis, employing gas chromatography with an FID detector and infrared spectroscopy to analyze the aliphatic and aromatic hydrocarbon fractions, respectively.

Microbial Transformation and degradation of toxic organic chemicals. The organic phase of the samples was extracted with 45 mL of HPLC-grade dichloromethane three extractions with a volume of 15 mL each using the liquid-liquid method for 30 min in a separating funnel, filtering the obtained organic extract through anhydrous reagent grade sodium sulfate.