Diazotization Titration or Nitrite Titration. Diazotization is used in the analysis of aromatic compounds containing an amino group in the. View Notes – from ECH at University of Florida. Diazotization titration (Nitrite Titration) Mr. V. M. Patil Assistant Professor & PG. Quantitative estimation of Eflornithine by Nitrite (Diazotization) titration Here the amino group of Eflornithine is diazotized with sodium nitrite solution in.
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Tittration ahead with the world’s most comprehensive technology and business learning platform. The diazotization titration is nothing but the conversion of the primary aromatic amine to a diazonium compound. An accurately weighed 1 mg sample of sulphonamide is dissolved in the 4 ml of concentrated HCl and in 10 ml of distilled water. What is the principle involved in the diazotization titrimetry?
For example, the aminophenol is readily oxidized by the nitrous acid and converted to the quinones in the presence of copper sulphate solution and forms the diazo oxide compounds.
Different amino compounds react with the nitrous acid titratioh different rates. Diazotization Titrations free pharmacy study material pharmaceutical analysis pharmaceutical analysis lecture notes. Then the solution is slowly titrated with the 0. Registration Forgot your password?
In non-aqueous titrations, usually, the milli volt scale of potentiometer is used rather than pH scale since the potential of the non-aqueous systems exceed the pH scale. The end point is determined by streaking one drop of the titration solution on the starch iodide paper until blue colour is appeared. This method is first used in the determination of dyes.
A Seminar on Potentiometric and Diazotization Titrations
Then this solution is titrated with the sodium nitrite solution. Used in the determination of the amphetamine. The titrafion procedure is followed by weighing the sample and transferring it into the standard flask.
They are as follows: Activity not concentration Concepts to review: Maintenance of the temperature is the main condition ciazotization the diazotization titration.
There ciazotization mainly three types of methods based on the titration procedure. Temperature conditions to be properly maintained throughout the reaction. The percentage amount of the sulpha drug is determined by the following equation: In Folin and Flanders titrated the acidic substances by using the non-aqueous solvents such as benzene, chloroform and chloroform-methanol mixture.
The steps involved in the non-aqueous titrimetric diazotisation are proposed by the Tomicek. The end point in diazotization titration is detected by the following procedures: Usually 30 — 50mV of potential is applied. The principle involved in this method is that the primary aromatic amine present in the sample reacts with the sodium nitrite in the presence of acid such as hydrochloric acid to obtain a diazonium salt.
Pharmaceutical Analysis by P. D. Chaithanya Sudha
The colour produced is not stable. The reaction mechanism was first proposed by Peter Griessin. The end point is detected by the formation of the blue colour with starch iodide paper. Used in the determination of the chloropheneramine. This method is mainly used for the titration of insoluble diazonium salts. At the end point, permanent deflection of the titraion is observed.
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Get unlimited access to videos, live online training, learning paths, books, tutorials, and more. The following conditions are required for the diazotization titration of the amino group containing samples.
4. Diazotization Titrations – Pharmaceutical Analysis [Book]
The indicator electrodes must readily come into equilibrium with one of the ions. Used in the determination of the P -amino benzoic acid vitamin B 4.
Then the electrodes are connected to the potentiometer and slowly titrated with sodium nitrite solution until a permanent deflection is observed at the end point. What are the example drugs assayed by the diazotization titrimetry? This process was first discovered in and was applied to the synthetic dye titrarion. Classified as solid state, heterogeneous, liquid ion exchanger and glass type. The liberated iodine is back titrated with the sodium thiosulphate using starch as the external indicator.
In this method, the primary aromatic amine is reacted with the sodium nitrite in acidic medium to form a diazonium salt. Potentiometric Electrodes Potentiometric electrodes measure: Hence the non-aqueous titrimetric method is used.
The accurately weighed sample is added to the 5 ml of HCl and 50 ml of distilled water.
A Seminar on Potentiometric and Diazotization Titrations – ppt video online download
What are the advantages of diazotization titrimetry? Relatively slow when compared to other methods. When sodium nitrite is reacted with the hydrochloric acid sodium chloride and nitrous acid are formed.
What is a chemical reaction?